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Its Lagging- free analog transmission shows activity in real- time and has a huge memory too. Important features at a glance: Provides crisp picture: Amcrest’s HD Over Analog (HDCVI) produces crisp and good resolutions to the surveillance industry, and there’s no struggle to identify crucial details in your footage and no gaps from lag in the feed. It provides high- resolution HD quality image. Remote viewing: The new generation of security systems changed the closed- circuit surveillance by making remote access a reality. By going beyond an internal UI, they have created unique and wonderful interfaces for remotely managing or seeing your 9. H system using common PC browsers or your smartphones. Day and night surveillance: It provides great picture quality during the day with 3. Also, it has switching features between streams and capturing screenshots. The app also has a playback option that helps to view recorded files with unlimited access by using your phone. The Pros and Cons of buying this Security camera are: Pros. Provides real- time video feeds with a crisp HD image unequaled by any other platforms in the private consumer market. It is easy to install. YAP is essential for tissue tension to ensure vertebrate 3. D body shape. Vertebrates have a unique 3. D body shape in which correct tissue/organ shape and alignment are essential for function. For example, vision requires the lens to be centred in the eye cup which must in turn be correctly positioned in the head. Tissue morphogenesis depends on force generation, force transmission through the tissue, and response of tissues and extracellular matrix (ECM) to force. Although a century ago D'Arcy Thompson postulated that terrestrial animal body shapes are conditioned by gravity. Here, we report a unique medaka mutant, hirame (hir), which is sensitive to deformation by gravity. We show that actomyosin- mediated tissue tension is reduced in hir embryos, leading to tissue flattening and misalignment, both of which contribute to body flattening. By analyzing YAP function in 3. D- spheroids of human cells, we identify the Rho. GAP ARHGAP1. 8 as an effector of YAP in controlling tissue tension. Together, these findings reveal a previously unrecognized function of YAP in regulating tissue shape and alignment required for proper 3. D body shape. Understanding this morphogenetic function of YAP could facilitate the use of embryonic stem cells to generate complex organs requiring correct alignment of multiple tissues. Via exhaustive mutant screening in medaka and zebrafish. Fig. Although general development was not delayed, hir mutants exhibited delayed blastopore closure (Fig. Fig. 1d), surviving until just before hatching (6 days post- fertilization, dpf). During body collapse, tissues and organs including neural tube and somites gradually became flattened and improperly aligned (Fig. Lenses were misaligned outside the eyes (Fig. Mutant lens placodes expressing sox. Fig. 1e. 1’, 2’, Extended Data Fig. Supplementary Videos 1, 2). These fragments gradually rounded up with some re- attaching to the retina to form ectopic lenses that were not incorporated (Fig. Thus, tissue flattening and misalignment defects are associated with the flattened mutant phenotype. Organ/tissue collapse and misalignment in hir mutants. Positional cloning identified a mutation of 1. Leu (TTG to TAG) in the WW1 domain of YAP in hir (Extended Data Fig. YAP is the nuclear executor of the Hippo pathway and regulates organ growth via stimulation of cell proliferation. In wild type (WT) embryos, YAP transcripts are ubiquitous throughout normal development. Medaka maternal YAP m. RNA was present at st. Extended Data Fig. Morpholino (MO) YAP knock- down (KD) in WT embryos recapitulated the hir phenotype (Extended Data Fig. Supplementary Table 1, 2), and ubiquitous recombinant YAP m. RNA expression rescued the hir phenotype (Extended Data Fig. In addition, perturbation of maternal YAP m. RNA translation in hir mutant embryos by YAP translation- blocking (TB) MO (m. YAP KD hir embryos) elicited a more severe blastopore closure and body flattening phenotype than in hir zygotic YAP mutants (Fig. Supplementary Table 2). Blastopore closure defects, but not flattening, have been reported in YAP KD zebrafish and Xenopus. Since TAZ is a functional paralog of YAP1. YAP KD phenotype in zebrafish. YAP; TAZ double KD zebrafish embryos exhibited more pronounced blastopore closure defects than YAP KD alone (Extended Data Fig. YAP- 4. SA, which lacks four serines and predominantly localizes to the nucleus. WT YAP (Extended Data Fig. YAP. The main nuclear function of YAP is to promote proliferation and inhibit cell death. Cell proliferation remained close to normal in hir embryos but was strongly suppressed in TAZ KD (and YAP/TAZ double KD) medaka embryos (Extended Data Fig. Thus, in medaka, cell proliferation is mainly regulated by TAZ, while YAP is predominantly required for 3. D body shape. 3 dpf hir mutants showed different orientations of body flattening. We therefore examined whether collapse correlated with the direction of gravity. Mutant embryos maintained either right- side or left- side down relative to the earth collapsed towards the earth as indicated by the ventricle tangent (Fig. Average collapse angle, . Mutant embryos maintained dorsal side down exhibited apparently uniform dorso- ventral compression (Fig. Thus, flattening in hir embryos reflects an inability to withstand external forces (i. During WT organogenesis, global levels of phosphorylated myosin regulatory light chain (p. MRLC), indicative of actomyosin activity, increased (Fig. To assess tissue tension during blastopore closure, we analyzed a surface epithelial cell layer, the enveloping layer (EVL)1. Extended Data Fig. Comparison of EVL shape anisotropy between WT and hir embryos suggested that tissue tension in hir is reduced within the EVL (Extended Data Fig. We also quantified actomyosin network tension within the yolk syncytial layer (YSL) of zebrafish embryos with compromised YAP function expressing EGFP myosin light chain protein, Tg(actb. GFP)1. 7. The YSL actomyosin network close to the EVL margin (Fig. AR) was cut along a 2. Recoil velocities were significantly reduced in YAP; TAZ KD (n=5. KD embryos (n=4. 0; 1. Consistent with this, epiboly movements in YAP; TAZ double KD zebrafish embryos were significantly reduced (KD embryos: 5. To test whether reduced actomyosin network tension is also responsible for neural tube tissue flattening in hir, we performed micropipette aspiration experiments. The higher deformability of hir neural tube tissue was paralleled when myosin activity was reduced by ROCK inhibition (Fig. Together, these analyses indicate that YAP is required for actomyosin- mediated tissue tension in medaka and zebrafish. Single cell tracking analysis of the growing neural tube in hir showed that tissue flattening was associated with failure to stack cells and increase in cells slipping to one side after perpendicular cell division (Fig. Extended Data Fig. Live imaging showed loss of filopodia between lens and retina which tether lens to retina during lens invagination. Extended Data Fig. The formation of lens- retina filopodia requires fibronectin (FN)- integrin signaling and contractile actomyosin. While st. 2. 2 WT embryos had elongated thin FN fibrils between invaginating lens and retina, hir retina showed punctate FN patches (Fig. FN fibril formation. In addition, large ectopic FN deposits were found on the retina in hir (Fig. Similar loss of normal FN fibrils and large FN deposits were observed throughout hir embryos (Fig. Furthermore, integrin . In contrast, cell- cell adhesion and apical markers, including pan- cadherin, atypical PKC (a. PKC) and ZO- 1, were unaltered in hir (data not shown). Mosaic expression of YAP in hir and transplantation experiments both showed that the hir mutation acts in a non- cell autonomous manner (Extended Data Fig. Supplementary Table 4). For instance, in invaginated hir lens rescued by mosaic expression of YAP, non- YAP expressing hir cells recovered filopodia (Extended Data Fig. These data suggest that YAP functions in tissue alignment by regulating FN assembly. Cell and tissue dynamics in hir mutants. To identify downstream YAP effectors regulating tissue tension, we used a human 3. D spheroid in vitro culture system employing the human retina pigmented epithelial cell line h. TERT- RPE1 (RPE1), which displayed a relatively mild proliferation defect upon YAP KD. YAP KD spheroids collapsed upon exposure to external forces by slow centrifugation, unlike normal spheroids (Fig. YAP maintains tissue tension also in human 3. D tissues. YAP KD spheroids also lacked the typical beehive- like pattern of FN fibrils and, instead, contained large FN deposits, reminiscent of the hir retina phenotype (Fig. Cortical actomyosin contraction is required for polymerizing FN monomers to form fibrils. Consistently, FN fibril formation on the basal surface of control spheroids coincided with cortical F- actin bundles (Fig. In contrast, loss of normal FN fibrils in YAP KD spheroids was associated with marked reduction of cortical F- actin bundles (Fig.
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Evangelos Kofos, author of a series of publications on the issue of the Macedonization of the Slav inhabitants of the FYROM. The borders got more confusing as time passed as the ancient Macedonians occupied and annexed more territories, almost exclusively over present day Bulgaria and even reaching the Danube River. Internally, the Macedonian Homeland was divided into Upper Macedonia and Lower Macedonia. Lower Macedonia included the rest of the country with the Axios and Strymon Valleys and the Khalkidiki Peninsula. Paeonians, and exclude the Greek states, one must question why not include the Thracians to the east and northeast of ancient Macedonia? Bitrakova- Grozdanova states, . Bitrakova- Grozdanova would not have made such a statement if she did not feel that the Macedonians were Greeks living south of Paeonia. Pangaeum and the Bottiaiei. Borza, agreeing with Hammond states, “First, the matter of the Hellenic origins of the Macedonians: Nicholas Hammond's general conclusion that the origin of the Macedonians lies in the pool of proto- Hellenic speakers who migrated out of the Pindus mountains during the Iron Age is acceptable. Slavs: New Invaders in Byzantium The Coming of the Slavs Compared to the history of their Hellenic and Albanian neighbors, the history of The Former Yugoslav Republic of Macedonia’s (FYROM) Slavs in the Balkansis very recent. It began approximately during the A. D. Timok well within modern Serbian Territory. The Slav masses concentrated in the Vardar Province (part of which is FYROM today), but they also existed in culturally separate communities elsewhere along with the much larger and historically entrenched Hellenic communities. Cyril and Methodius, Equal to the Apostles, Illuminators of the Slavs. The Encyclical promulgated on 2. November 1. 90. 1 (On the Foundation of a Seminary in Athens, Pope Leo XIII). Apostolic Letter Pacis Nuntius(3) of 2. October 1. 96. 4, proclaimed Saint Benedict Patron of Europe. Egregiae Virtutis, by Pope John Paul II, December 3. Encyclical Epistle Grande Munus (3. September 1. 88. 0), in Leonis XIII Pont. Acta, II, PP. 1. 25 1. Cyrillum (1. 3 February 1. Archbishops and Bishops of the Kingdom of the Serbs- Croats- Slovenes and of the Czechoslovakian Republic: AAS 1. JOHN XXIII, Apostolic Letter Magnifici Eventus (1. May 1. 96. 3) to the Prelates of the Slav Nations: AAS 5. PAUL VI, Apostolic Epistle Antiquae Nobilitatis (2 February 1. Saint Cyril: AAS 6. UT UNUM SINT (That They May Be One) Pope John Paul II, 2. May 1. 99. 5. Cyril and Methodius University in Veliko T. Petar Djordjic states . Cyril and Methodius was Greek. Nevertheless, there is a plethora of statements found in mostly Slavic websites and books written by Slavs offering a variety of statements about Maria’s nationality, from . Accepting the assumption that Maria was a Slav, we have to presuppose that she was an Orthodox Christian before she married her husband Leon, since the Christian Church and especially the Greek Orthodox Church even today, does not permit marriages between Christians and non- Christians. Methodius was the elder brother and his baptismal name was probably Michael. Although Methodius was older, Cyril became more eminent, mainly because ofhis knowledge of the Slavic language, which he had acquired earlier. Like all children of the higher imperial officials, the two brothers received their advanced education at the Imperial School of Constantinople. Archon or Prefect) of the Strymon District of Macedonia, but more possibly in Bithynia,6. Slavs had been resettled. The decline of the First Bulgarian Empire. On July 2. 9, 1. 01. Belasica (Greek: Kleidion) close to Strumica (present day The FYROM), the Emperor of Byzantium Basil II of the Macedonian dynasty, ended Samuil's empire by capturing 1. Samuel was not able to resist openly, nor to face the emperor in open warfare, so, weakened from all sides, he came down from his lofty lair to fortify the entrance to Bulgaria with ditches and fences. A wall was built across the whole width . When the emperor had thus despaired of gaining passage, Nikephoros Xiphias, the strategos of Philippopolis, met with the emperor and urged him to stay put and continue to assault the wall, while, as he explained, he turned back with his men and, heading round to the south of Kleidion through rough and trackless country, crossed the very high mountain known as Belasica. The Bitola Inscription is an inscription made by order of Bulgarian Tsar Ivan Vladislav in 1. Bitola fortress. The Ilinden Uprising Six Bulgarian intellectuals, Hristo Tatarchev, Damian Gruev, Ivan Hadzhinikolov, Petar Poparsov, Andon Dimitrov, and Hristo Batandzhiev, striving to obtain civil rights for the Bulgarian population in Macedonia and Thrace (regions cut off from Bulgaria and left under the domination of the Ottoman sultans), founded the Internal Macedonian- Adrianople Revolutionary Organization (IMRO, VMRO in Bulgarian) on October 2. Thessaloniki (presently the capital of the Greek Macedonia). Clark, and Helen . The following are excerpts of how modern scholars and newspapers of the era viewed the Ilinden Uprising as a Bulgarian uprising. Duncan Perry states, . Our aim, our ideal is autonomy for Macedonia and the Adrianople region, and we must also bring into the struggle the other people who live in these two provinces as well.. We the Bulgarians of Macedonia and Adrianople, must not lose sight of the fact that there are other nationalities and states who are vitally interested in the solution of this question. Any intervention by Bulgaria would provoke intervention by neighbouring states as well, and could result in Macedonia being torn apart. Prochaska, to the Foreign Ministry on the situation in Macedonia after the Ilinden Uprising, dated November 2. The Bulgarian rebel leader, Damyan Grouev, was detained by the Serbian rebel detachment of Mitsko for several weeks in the region of Porech, but he was later released, and returned to Bulgaria through Skopje.” 7. Damyan Gruev was one of the leaders of the Ilinden Uprising. Brailsford who wrote about Macedonia and its people, dedicated Chapter V of his book to the Ilinden Uprising of 1. Bulgarian movement. The Treaty of Bucharest of 1. In July of 1. 90. Borders of the Balkan Countries as of 1. August 1. 91. 3 It is routinely publicized that the Peace Treaty of Bucharest split . First Assumption: the Existence of the . Laffan explains: By . The Protocol of Florence among the great powers (England, France, Austria, Russia, Germany, and Italy) dated 1. December 1. 91. 3 was concerned with the borders of the newly formed State of Albania. The Peace Treaty of Neuilly dated 2. Torrentz Search Engine. Torrentz will always love you. We have a wide choice. Nokia Symbian applications Mobile cell Quickoffice N95 3rd Edition Agile SMS Answering Blacklist S60 smartmovie Opera Epocware Nemesis Pocket Dictionary Oxford. Garmin N95 8gb Download ItunesIf you’ve always wanted a way to download an application from the Android Market directly to your PC, boy do we have the right thing for you. An Android developer. Gowdy # If you have any new entries, please submit them via # http:// # or send. Torrentz will always love you. If having trouble with update not being received by your device. 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